منابع مشابه
A packaging cell line for lentivirus vectors.
Lentivirus vectors can transduce dividing and nondividing cells. Using three-plasmid transient transfections, high-titer (>10(9) IU/ml) recombinant lentivirus vectors pseudotyped with vesicular stomatitis virus G (VSV-G) protein can be generated (T. Kafri et al., Nat. Genet. 17:314-317, 1997; H. Miyoshi et al., Proc. Natl. Acad. Sci. USA 94:10319-10323, 1997; L. Naldini et al., Science 272:263-...
متن کاملDevelopment of multigene and regulated lentivirus vectors.
Previously we described safe and efficient three-component human immunodeficiency virus type 1 (HIV-1)-based gene transfer systems for delivery of genes into nondividing cells (H. Mochizuki, J. P. Schwartz, K. Tanaka, R. O. Brady, and J. Reiser, J. Virol. 72:8873-8883, 1998). To apply such vectors in anti-HIV gene therapy strategies and to express multiple proteins in single target cells, we ha...
متن کاملGeneration of domestic transgenic cloned kittens using lentivirus vectors.
The efficient use of somatic cell nuclear transfer (SCNT), in conjunction with genetic modification of donor cells provides a general means to add or inactivate genes in mammals. This strategy has substantially improved the efficacy of producing genetically identical animals carrying mutant genes corresponding to specific human disorders. Lentiviral (LV) vectors have been shown to be well suite...
متن کاملNondividing cells: a safer bet for integrating vectors?
efficient in penetrating tumor tissues as compared with antibodies, and the results confirm that only three injections of T cells are necessary for complete tumor regression. However, only a comparative study using antibodies and engineered T cells will validate this claim. Patients treated with CAR-engineered T cells could also develop resistance similar to that in lymphoma patients in whom CD...
متن کاملTowards safer vectors for the field release of recombinant bacteria.
The prospect of the deliberate environmental release of genetically manipulated microorganisms has given rise to a great deal of polemic. Amongst the rational scientific concerns are those concerned with the fate of the released bacteria, the fate of the recombinant genes that they carry, the selective pressures acting upon them in different environmental situations and the long term effects on...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2007
ISSN: 1525-0016
DOI: 10.1038/sj.mt.6300111